Flow Cytometry Protocols, T.s.hawley, R.g.hawley

This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers

Flow Cytometry of Hematological Malignancies
Flow cytometric analysis is often integral to the swift and accurate diagnosis of leukemias and lymphomas of the blood, bone marrow, and lymph nodes. However, in the fast-moving and expanding field of clinical hematology, in can be challenging to remain up to speed with the latest biological research and technological innovations. Flow Cytometry of Hematological Malignancies has been designed to provide all those working in hematological oncology with a practical, cutting-edge handbook, featuring clear and fully illustrated guidance on all aspects of cytometry’s role in diagnosis and analysis. This essential second edition includes:

This volume provides protocols to successfully apply cutting-edge technologies to characterize the biology of T cells at an unprecedented level of complexity. Chapters guide readers through flow cytometry and fluorescence-activated cell sorting, the behaviour of single T cells after adoptive cell transfer (ACT), single cell gene expression by multiplex PCR, lentiviral transduction approaches, protocols to derive large numbers of early-differentiated memory T cells by using dedicated cytokines cocktails, approaches to measure telomerase activity in terminally differentiated T cells, and approaches to define Treg cells at the phenotypic and functional level.

Richard Hawley is widely recognised as both one of the UK’s greatest songwriters and one of its most respected. Alongside two Mercury Music Prize Nominations, a BRIT award nomination and 4 UK Top 10 Albums, the Sheffield native songwriter has collaborated with the likes of Pulp, Arctic Monkeys, Lisa Marie Presley, Paul Weller and Elbow. Following the success of Standing At The Sky’s Edge, which won Best New Musical and handed Hawley Best Original Score at the 2023 Olivier Awards, BMG announce the first ever Richard Hawley collection; Now Then: The Very Best of Richard Hawley. Curated by Hawley and long-term collaborator Colin Elliot, Now Then spans his twenty plus year career, exhibiting all facets of his song writing mastery; marrying his most cinematic moments with his most tender, his best loved work and a host of hidden gems from his early work, to create a collection for both his hardcore fanbase and the perfect entry point for his newest fans. Includes tracks from Richard Hawley (2001), Late Night Final (2001), Lowedges (2003), Coles Corner (2005), Lady’s Bridge (2007), Truelove’s Gutter (2009), Standing at the Sky’s Edge (2012), Hollow Meadows (2015) and Further (2019)

Groupe et orchestre studio du mythique label Stax, ce quartette qui représente un véritable melting pot culturel, mêlant musiciens afro-américains élevés au sonorités R&B et au gospel, et musiciens blancs issus des classes défavorisées, amateurs de rockabilly et country, naît officiellement en 1962 et symbolise le « Memphis Sound », ce rythm’n’blues « sudiste » qui illuminera les années 60, porté par des artistes incontournables tels que Otis Redding, Sam & Dave, Rufus et Carla Thomas, The Staple Singers, Wilson Pickett ou encore Isaac Hayes.