Laboratory Manual For Biotechnology And Laboratory Science

Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines.

Transgenic plants serve as essential tools for research in plant biology and are the basis of modern agricultural biotechnology. In Transgenic Plants: Methods and Protocols, leading scientists describe readily reproducible techniques they have perfected for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems, including Agrobacterium tumefaciens, Agrobacterium rhizogenes, particle bombardment, electroporation, floral-dip, and chloroplast transformation, as well as the regeneration of whole transgenic plants by organogenesis and somatic embryogenesis from different explant cells/tissues and from such diverse plant species as tomato, cassava, conifers, and citrus.

Elevate Your Laboratory Expertise: GLCP Mastery Course Certification and Beyond

By Allison Hubel

Understanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years.