Model Systems of Development And Aging of The Nervous System

This volume discusses different approaches towards understanding the brain plasticity, aging, and regeneration of the nervous system in model organisms. Chapters guide the reader through an overview of model organisms, setup of an aquatic system, Tol2-mediated transgenesis, CRISPR/Cas9-mediated gene knock-out and knock-in, Cas13d-mediated gene knockdown, tissue clearing and expansion methods for brain and spinal cord imaging, Histological techniques, chromatin Immunoprecipitation Sequencing (ChIP-Seq) analysis, single-cell RNA sequencing analysis, BrdU/EdU incorporation assays, brain and spinal cord injury in African killifish, and emerging technologies and future perspectives in African killifish research. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Learn how to easily design and implement a software system using object-oriented programming. Through hands-on activities, this book equips you with the real-world practice needed to master OOP skills that can be applied universally across any programming language.

Problems with how the neurotransmitter glutamate functions in the brain have been linked to a wide variety of disorders, including schizophrenia, Alzheimer's, substance abuse, and traumatic brain injury. Efforts to understand, treat, and prevent glutamate-related disorders can be aided by the identification of valid biomarkers.

Most scientists agree that the 21st century will be the "Century of Biology". The revolution that started a few decades ago with the extraordinary develop­ ment of molecular biology, has provided the basis for the understanding of the mechanisms that govern life through the regulation of cell function and cell-to­ cell interactions. Neuroscience will surely be one of the biological disciplines that will profit the most from this revolution. Neuroscience comprises the study of the brain, and the more difficult relationship of brain and behavior.

Corona- and related viruses are important human and animal pathogens that also serve as models for other viral-mediated diseases. Interest in these pathogens has grown tremendously since the First International Symposium was held at the Institute of Virology and Immunobiology of the University of Wiirzburg, Germany. The Sixth International Symposium was held in Quebec City from August 27 to September I, 1994, and provided further understanding of the molecular biology, immunology, and pathogenesis of corona-, toro-, and arterivirus infections.