Phaidon Editors

This volume explores base editors (BEs), an invaluable CRISPR-based genome editing tool with a wide variety of versatile applications. Beginning with an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for disease modeling in mammalian cells and generating mutagenic mice, therapeutic base editing strategies, which covers delivery methods of BE-encoded DNA plasmids, mRNAs, or ribonucleoproteins through viruses or non-viral lipid nanoparticles, and lastly, the use of BEs in plants and bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

"In This Light and on This Evening" is the third studio album by English rock band Editors. The band had said the material would have a more raw and anthemic sound compared to their previous work. Following its release, the album debuted at number one on the UK Albums Chart. In 2012 it was awarded a double gold certification from the Independent Music Companies Association.

"The Weight of Your Love" is the follow up to Editors' two consecutive Number One UK albums, 2007's 'An End Has a Start' and 2009's 'In This Light And on This Evening'. Their debut album, 2005's "The Back Room" has sold over half a million copies in the UK. This is the first album to feature new members Justin Lockey and Elliott Williams, following the departure of founding member Chris Urbanowicz in 2012. It was recorded at Blackbird Studio in Nashville with producer Jacquire King (Tom Waits, Norah Jones, of Monsters And Men, Kings of Leon) and mixed by Craig Silvey (Arctic Monkeys, the Horrors, Arcade Fire, Bon Iver).

Video editors often need to switch to Adobe After Effects for compositing work, such as custom lower-third for displaying captions and text in their projects. After Effects has all the tools you need for the job, and renowned expert Kevin P McAuliffe is here to show you how it all works.