Recombinant Protein Expression in e Coliin

Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your guide through the jungle of new methods and techniques available to analyse gene expression - the first to provide such a broad view of the measurement of mRNA and protein expression in vitro, in situ and even in vivo. Despite this broad approach, detail is sufficient for you to grasp the principles behind each method. In each case, the authors weigh up the advantages and disadvantages, paying particular attention to the automated, high-throughput processing demanded by the biotech industry. Completely up to date, the book covers such ground-breaking methods such as DNA microarrays, *****l analysis of gene expression, differential display, and identification of open reading frame expressed sequence tags. All the methods and necessary equipment are presented visually in more than 300 mainly colour illustrations to assist their step-by-step reproduction in your laboratory. Each chapter is rounded off with its own set of extensive references that provide access to detailed experimental protocols. In short, the bible of analysing gene expression.

Protein Crystallography in Drug Discovery - 2004 - Robert E Babine & Sherin Abdel-Meguid - 3527306781
Protein Crystallography in Drug Discovery (Methods and Principles in Medicinal Chemistry)
Author: Robert E. Babine / Sherin S. Abdel-Meguid / Raimund Mannhold / Hugo Kubinyi / Gerd Folkers
pages: 278 pages Published Online: 19 Feb 2004
Publisher: John Wiley & Sons
ISBN: 3527306781
Format: pdf
Size: 4.9 MB

Non-vesicular intracellular cholesterol transport is an important mechanism for maintaining membrane cholesterol homeostasis. Recent reports of studies directed at soluble cholesterol transport proteins indicate that aberrant expression of the START proteins may contribute to disease states associated with disorders in cholesterol homeostasis.