Next Generation Sequencing

This book reviews the potential of next-generation sequencing (NGS) in research on and management of colorectal cancer (CRC), a leading cause of death worldwide and one of the most biologically and clinically heterogeneous cancers.

This book written by experts covers various aspects of Next generation sequencing (NGS) including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. The book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.

Tag-based approaches were originally designed to increase the throughput of capillary sequencing, where concatemers of short sequences were first used in expression profiling. New Next Generation Sequencing methods largely extended the use of tag-based approaches as the tag lengths perfectly match with the short read length of highly parallel sequencing reactions.

by Wei Wu (Editor), Hani Choudhry (Editor)