Recombinant Protein Production in Plants

This volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression. Chapters guide readers through recombinant protein production in important plant systems, protein recovery and purification, different strategies to optimise productivity, cloning and fusion protein approaches, and the regulation and freedom to operate analysis of plant-produced proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

The recent achievements in engineering studies on plant cell cultures are reviewed, included are the gas concentration effects and bioprocess integration for the enhanced productivity of plant secondary metabolites. The metabolic engineering of plant secondary metabolite pathways and recombinant protein production from genetically modified plant cells are introduced. Large-scale plant micropropagation via somatic embryogenesis and hairy roots is discussed for efficient propagation of desease-free, genetically uniform and massive amounts of plants in vitro in massive amounts. Characterization and application of hairy plant roots endowed with photosynthetic functions is also covered in this special volume.