Human Cell Culture

Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement.

Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement.

Expert scientific and clinical investigators present proven human cell culture techniques applicable to tissue samples taken from a wide variety of organs, particularly those prone to pathological change. They describe in clear, step-by-step instructions the special requirements for successfully culturing such human cells as T-cells, trophoblast cells, renal cells, natural killer cells, endothelial cells, neurons, epithelial cells, pituitary cells, and more.

This unique comprehensive volume in the Human Cell Culture Series encompasses research methodology for the growth and differentiation of all types of primary hematopoietic cells. Each of the twelve hematopoietic cell types is covered by a leading expert in the field, providing insightful background information along with detailed current culture and assay techniques. In addition to uses for research applications, current and future clinical applications of large-scale culture methods are also discussed.

If you wish to grow or characterize embryonic stem cells or persuade them to differentiate into a particular cell type, then this book contains information that is vital to your success. The aim is to provide clear simple instructions and protocols for growing, maintaining and characterizing embryonic stem cells and details of the various methods used to make stem cells differentiate into specific cell types. The contents will be of interest to stem cell biologists, tissue engineers and scientists wishing to use embryonic stem cells for therapeutic purposes. Each chapter has been written and edited by internationally respected scientists working at the cutting edge of technological developments in human embryonic stem cells.