Singlemolecule Fluorescence Spectroscopy of The Folding of a Repeat Protein

by Sharona Cohen (Author)
Nominated as an Outstanding Ph.D. thesis by Weizmann Institute of Science, Rehovot, Israel
First optical characterization of elasticity of a repeat protein
Comprehensive single-molecule FRET experiments probe both dynamics and structure during equilibrium folding

This book is devoted to the physical and mathematical modeling of the formation of complexes of protein molecules. The models developed show remarkable sensitivity to the amino acid sequences of proteins, which facilitates experimental studies and allows one to reduce the associated costs by reducing the number of measurements required according to the developed criteria. These models make it possible to reach a conclusion about the interactions between different amino acid chains and to identify more stable sites on proteins. The models also take the phosphorylation of amino acid residues into account.

Philosophers have warned of the perils of a life spent without reflection, but what constitutes reflective inquiry - and why it's necessary in our lives - can be an elusive concept.