Digital Pcr

This volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

This volume is dedicated to tropical diseases. Advances in the understanding of neglected tropical diseases (NTDs) are inspiring the scientific community to investigate their eco-epidemiology and physiopathology, and to employ new biotechnological tools to fight against these devasting and complex infectious diseases which are associated with poverty.

This volume is dedicated to tropical diseases. Advances in the understanding of neglected tropical diseases (NTDs) are inspiring the scientific community to investigate their eco-epidemiology and physiopathology, and to employ new biotechnological tools to fight against these devasting and complex infectious diseases which are associated with poverty.

This volume is dedicated to tropical diseases. Advances in the understanding of neglected tropical diseases (NTDs) are inspiring the scientific community to investigate their eco-epidemiology and physiopathology, and to employ new biotechnological tools to fight against these devasting and complex infectious diseases which are associated with poverty.

This volume provides a thorough guide of the study of multiple myeloma (MM)—from sample acquisition to the performance of molecular tests, and also discusses difficulties that arise during quality control and interpretation of results. The chapters in this book cover topics, such as next-generation-flow-based methods for detection of minimal residual disease and circulating tumor cells; Cytometry by Time-of-Flights (CyTOF); a droplet digital PCR approach for finding contamination in patients’ samples; genome-wide investigation of the methylation and hydroxymethylation status of cytosines; and a microfluidic approach for creating nanoparticles. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.